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hacat cells  (ATCC)


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    ATCC hacat cells
    In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of <t>HaCaT</t> <t>cells</t> treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.
    Hacat Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hacat+cells/HEK001/pmc13273585-214-14-19
    Average 95 stars, based on 127 article reviews
    hacat cells - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "G-quartet driven adaptive hydrogels that integrate constitutional dynamics for antibacterial action and tissue repair"

    Article Title: G-quartet driven adaptive hydrogels that integrate constitutional dynamics for antibacterial action and tissue repair

    Journal: iScience

    doi: 10.1016/j.isci.2026.116107

    In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of HaCaT cells treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.
    Figure Legend Snippet: In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of HaCaT cells treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.

    Techniques Used: In Vitro, In Vivo, Wound Healing Assay, Migration, Immunofluorescence, Staining, Control, Comparison

    Related Articles

    Cell Culture:

    Article Title: Large scale semi-automated quantification of viral spread reveals multi-phasic structure of plaque formation
    Article Snippet: .. All cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% foetal bovine serum (FBS, Australian origin, Bovogen) for HaCaT cells (ATCC, CCL-2) and 5% for BS-C-1 cells (ATCC, CCL-26). .. Additionally, 1% PSG (Gibco), containing 100 units/mL penicillin, 100 μg/ml streptomycin, and 292 μg/ml L-glutamine, was added.

    Article Title: Halomonas fermentation promoted the biotransformation of active substances from Dictyophora indusiata pileus and the enhancement of antioxidant capacity
    Article Snippet: Dictyophora indusiata (D. indusiata), a valuable medicinal and edible mushroom, is known for its numerous health benefits, but its pileus is often overlooked.. In this study, we utilized Halomonas species TD01 (Halomonas sp. TD01) to ferment extracts of D. indusiata pileus to facilitate the release of bioactive components and enhance antioxidant activity.. The results showed that Halomonas sp. TD01 effectively converted the extracts into a series of active metabolites.

    Article Title: Autocrine IL-8 Contributes to Propionibacterium Acnes-induced Proliferation and Differentiation of HaCaT Cells via AKT/FOXO1/ Autophagy.
    Article Snippet: [Abstract] Objective: Proprionibacterium acnes (P. acnes)-induced inflammatory responses, proliferation and differentiation of keratinocytes contribute to the progression of acne vulgaris (AV).. P. acnes was found to enhance the production of interleukin-8 (IL-8) by keratinocytes.. This study aimed to investigate the role of IL-8 in P. acnes-induced proliferation and differentiation of keratinocytes and the underlying mechanism.

    Article Title: Ginsenoside Rb1-engineered nanocomposite hydrogel promotes pressure injury repair through SIRT1-AMPK-mediated ferroptosis inhibition and angiogenesis activation
    Article Snippet: .. Before experimentation, HaCaT cells (PCS-200-011; ATCC, USA) were cultured for 18 h in DMEM medium (D0822; Merck, USA) containing a standard glucose concentration (4.5 mg/mL, equivalent to 25 mM). ..

    Modification:

    Article Title: Large scale semi-automated quantification of viral spread reveals multi-phasic structure of plaque formation
    Article Snippet: .. All cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% foetal bovine serum (FBS, Australian origin, Bovogen) for HaCaT cells (ATCC, CCL-2) and 5% for BS-C-1 cells (ATCC, CCL-26). .. Additionally, 1% PSG (Gibco), containing 100 units/mL penicillin, 100 μg/ml streptomycin, and 292 μg/ml L-glutamine, was added.

    Article Title: Autocrine IL-8 Contributes to Propionibacterium Acnes-induced Proliferation and Differentiation of HaCaT Cells via AKT/FOXO1/ Autophagy.
    Article Snippet: [Abstract] Objective: Proprionibacterium acnes (P. acnes)-induced inflammatory responses, proliferation and differentiation of keratinocytes contribute to the progression of acne vulgaris (AV).. P. acnes was found to enhance the production of interleukin-8 (IL-8) by keratinocytes.. This study aimed to investigate the role of IL-8 in P. acnes-induced proliferation and differentiation of keratinocytes and the underlying mechanism.

    Concentration Assay:

    Article Title: Ginsenoside Rb1-engineered nanocomposite hydrogel promotes pressure injury repair through SIRT1-AMPK-mediated ferroptosis inhibition and angiogenesis activation
    Article Snippet: .. Before experimentation, HaCaT cells (PCS-200-011; ATCC, USA) were cultured for 18 h in DMEM medium (D0822; Merck, USA) containing a standard glucose concentration (4.5 mg/mL, equivalent to 25 mM). ..



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    In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of HaCaT cells treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.

    Journal: iScience

    Article Title: G-quartet driven adaptive hydrogels that integrate constitutional dynamics for antibacterial action and tissue repair

    doi: 10.1016/j.isci.2026.116107

    Figure Lengend Snippet: In vitro and in vivo evaluation of G4TZ hydrogel in wound healing (A) Scratch wound healing assay of HaCaT cells treated with guanosine (G) gel and G4TZ gel. Representative images were captured at 0-, 6-, and 12-h post-wound creation to assess cell migration and wound closure. (B) Ki67 immunofluorescence staining reveals keratinocyte proliferation in HaCaT cells. Representative images of untreated (control, top panel) and G4TZ treated (bottom panel) HaCaT cells, where Ki67-positive nuclei (green) indicate proliferating cells. Scale bars, 25 μm. (C) Schematic illustration of G4TZ-mediated wound healing. (D) In vivo wound healing evaluation. Top representation, images of wounds of control groups on days 0, 3, 5, 8 and 12. Bottom panel, images of wounds of G4TZ treated groups on days 0, 3, 5, 8, and 12. (E) Histological comparison of skin wound healing of untreated and G4TZ treated mice skin. Representative H&E-stained skin sections showing (A) healthy skin architecture with intact epidermis, dermis, and visible skin appendages such as hair follicles and sebaceous glands. (B) Untreated wound area displays disorganized tissue architecture, absence of epidermal layer, and dense inflammatory cell infiltration. (C) G4TZ treated wounded skin at a later stage of healing, displaying mature granulation tissue, reduced cellularity, and enhanced collagen deposition indicative of early scar formation. Scale bars, 25 μm.

    Article Snippet: WRL68 cells were obtained from National Centre for Cell Science (NCCS), Pune, India, and HaCaT cells were obtained from ATCC (Cat# CRL-2404).

    Techniques: In Vitro, In Vivo, Wound Healing Assay, Migration, Immunofluorescence, Staining, Control, Comparison